Phosphate dilution lowers net phosphatidyl choline synthesis in lymphocytes

The rate of net phosphatidyl choline (PC) synthesis in lymphocytes was evaluated, under appropriate conditions, in terms of [32P]phosphate incorporation. Phosphate-diluted and normal media were compared, with the cells being stimulated by concanavalin A or not. In either case, incubation in phosphate-diluted medium lowered net PC synthesis. In concanavalin A-stimulated cells, phosphate dilution also abolished the stimulation effect on net PC synthesis in G1. Nevertheless, these changes did not inhibit blastogenic transformation of cells.