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Dieckol inhibits non-small-cell lung cancer cell proliferation and migration by regulating the PI3K/AKT signaling

Chun-Hong Wang1, Xiao-Feng Li1, Li-Fang Jin1

  • 1Department of Oncology and Hematology, The Second Hospital of Jilin University, Changchun, Jilin, China.

Journal of Biochemical and Molecular Toxicology
|July 11, 2019
PubMed

Insights

Dieckol, a compound from brown algae, effectively inhibits non-small-cell lung cancer (NSCLC) cell invasion and migration. It induces apoptosis by impacting the PI3K/AKT/mTOR pathway, suggesting its potential as a natural anticancer drug for NSCLC.

Area of Science:

  • Pharmacology
  • Oncology
  • Natural Products Chemistry

Background:

  • Non-small-cell lung cancer (NSCLC) presents a significant global health challenge with high mortality rates.
  • Dieckol, a polyphenol from brown algae, exhibits known antioxidant and anti-inflammatory effects.

Purpose of the Study:

  • To investigate the anticancer properties of dieckol against the A549 NSCLC cell line.
  • To elucidate the molecular mechanisms underlying dieckol's anti-cancer activity.

Main Methods:

  • Cytotoxicity assessed via MTT assay (LC50 = 25 µg/mL).
  • Apoptosis evaluated using acridine orange/propidium iodide (AO/PI) and Annexin FITC/JC-1 staining via flow cytometry.
  • Invasiveness assessed using Transwell assays.
  • Molecular mechanisms explored through caspase level determination and immunoblotting of the PI3K/AKT/mTOR pathway.

Main Results:

  • Dieckol demonstrated significant inhibition of A549 cell invasion and migration.
  • Dieckol induced apoptosis in NSCLC cells.
  • The compound suppressed the PI3K/AKT/mTOR signaling pathway and activated the tumor suppressor E-cadherin.

Conclusions:

  • Dieckol exhibits potent anticancer activity against NSCLC cells.
  • Dieckol functions as an anti-invasive and pro-apoptotic agent by modulating key cellular pathways.
  • Dieckol represents a promising natural compound for the development of novel NSCLC therapeutics.
Keywords:
NSCLCPI3K/AKT/mTORanticancer drugcadherindieckollung cancer

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