Insulin-like peptides involved in photoperiodism in the aphid Acyrthosiphon pisum

Aphids were the first animals reported as photoperiodic as their life cycles are strongly determined by the photoperiod. During the favourable seasons (characterised by long days) aphid populations consist exclusively of viviparous parthenogenetic females (known as virginoparae). Shortening of the photoperiod in autumn is perceived by aphids as the signal that anticipates the harsh season, leading to a switch in the reproductive mode giving place to the sexual morphs (oviparae females and males) that mate and lay winter-resistant (diapause-like) eggs. The molecular and cellular basis governing the switch between the two reproductive modes are far from being understood. Classical experiments identified a group of neurosecretory cells in the pars intercerebralis of the aphid brain (the so called group I of neurosecretory cells) that were essential for the development of embryos as parthenogenetic females and were thus proposed to synthesise a parthenogenesis promoting substance that was termed "virginoparin". Since insulin-like peptides (ILPs) have been implicated in the control of diapause in other insects, we investigated their involvement in aphid photoperiodism. We compared the expression of two ILPs (ILP1 and ILP4) and an Insulin receptor coding genes in A. pisum aphids reared under long- and short-day conditions. The three genes showed higher expression in long-day reared aphids. In addition, we localised the site of expression of the two ILP genes in the aphid brain. Both genes were found to be expressed in the group I of neurosecretory cells. Altogether, our results suggest that ILP1 and ILP4 play an important role in the control of the aphid life-cycle by promoting the parthenogenetic development during long-day seasons while their repression by short days would activate the sexual development. Thus we propose these ILPs correspond to the so called "virginoparin" by early bibliography. A possible connection with the circadian system is also discussed.