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Long non-coding RNA SNHG5 promotes glioma progression via miR-205/E2F3 axis

Xiaojian Li1, Liang Liu1, Yidan Luo2

  • 1Department of Neurosurgery, Nanjing First Hospital, Nanjing Medical University, Nanjing 210006, China.

Bioscience Reports
|July 12, 2019
PubMed

Insights

Small nucleolar RNA host gene 5 (SNHG5) promotes glioma progression by sponging miR-205 and targeting E2F transcription factor 3 (E2F3). This SNHG5/miR-205/E2F3 axis offers a potential therapeutic target for glioma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) show abnormal expression in tumors, but their precise mechanisms in glioma are largely unknown.
  • Small nucleolar RNA host gene 5 (SNHG5) is implicated in various cancers, yet its role in glioma requires elucidation.

Purpose of the Study:

  • To investigate the molecular mechanism of SNHG5 in glioma progression.
  • To explore the potential of the SNHG5/miR-205/E2F3 pathway as a therapeutic target for glioma.

Main Methods:

  • Quantitative real-time PCR to assess SNHG5 expression in glioma tissues and cell lines.
  • Cellular assays to evaluate the impact of SNHG5 on glioma cell glucose uptake, migration, and invasion.
  • RNA immunoprecipitation and luciferase reporter assays to confirm the interaction between SNHG5, miR-205, and E2F3.
  • Western blotting to detect E2F3 protein levels.
  • Xenograft mouse models to validate the role of SNHG5 in glioma tumorigenesis in vivo.

Main Results:

  • SNHG5 expression is significantly upregulated in glioma tissues and correlates with increased glucose uptake, migration, and invasion.
  • SNHG5 functions as a molecular sponge for miR-205, inhibiting its tumor-suppressive activity.
  • miR-205 directly targets E2F transcription factor 3 (E2F3), and SNHG5 overexpression leads to increased E2F3 levels.
  • Silencing SNHG5 inhibits glioma tumor growth in vivo.

Conclusions:

  • The SNHG5/miR-205/E2F3 axis plays a crucial role in promoting glioma progression.
  • Targeting the SNHG5/miR-205/E2F3 pathway presents a promising therapeutic strategy for glioma treatment.
Keywords:
E2F3SNHG5ceRNAgliomalarge intervening non-coding RNA

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