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Updated: Jan 22, 2026
07:52
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
Published on: April 26, 2018
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Targeting VPAC1 Receptors for Imaging Glioblastoma.
Sushil K Tripathi1, Rhonda Kean2, Emily Bongiorno2
1Department of Radiology, Thomas Jefferson University, Philadelphia, PA, USA.
Molecular Imaging and Biology
|July 12, 2019
Summary
This study shows that [64Cu]TP3805 can effectively target VPAC1 receptors for positron emission tomography (PET) imaging of malignant glioblastoma (MG) in mice. The novel tracer demonstrated high tumor uptake and a favorable tumor-to-normal brain ratio, enabling clear visualization of tumors.
Area of Science:
- Oncology
- Radiochemistry
- Molecular Imaging
Background:
- Malignant glioblastoma (MG) imaging presents challenges.
- VPAC1 receptors are potential targets for glioblastoma detection.
Purpose of the Study:
- To evaluate VPAC1-specific peptide [64Cu]TP3805 for positron emission tomography (PET) imaging of orthotopic MG in a mouse model.
Main Methods:
- VPAC1 expression was confirmed in glioma cell lines.
- Cell-binding assays and receptor-blocking studies validated [64Cu]TP3805 specificity.
- Micro-PET/CT imaging and tissue distribution analysis were performed in mice with orthotopically implanted GL261 tumors.
Main Results:
- [64Cu]TP3805 showed high binding affinity to VPAC1-expressing cells.
- PET imaging revealed significant [64Cu]TP3805 accumulation in MG tumors with a high tumor/normal brain ratio (8.1 ± 1.1).
- Histology and digital autoradiography confirmed PET findings, differentiating tumors from healthy brain tissue.
Conclusions:
- Targeting VPAC1 receptors with [64Cu]TP3805 is a promising approach for PET imaging of MG.
- Further investigation is warranted to explore the clinical potential of this novel imaging agent.
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