One-Pot, Multicomponent Strategy for Designing Lymphoseek-Inspired Hetero-Glycoadjuvant@AuNPs

Searching for vaccines (antigen and adjuvant) with easy preparation and strong T-cell response are crucial for antitumor immunity. In this work, to design lymphoseek-inspired vaccine possessing the abilities of promoting vaccine internalization and enhancing CD8⁺ T-cell responses, a simple multicomponent strategy is successfully utilized to fabricate lymph node and dendritic cell dual-targeting glycoadjuvant@AuNPs in one pot, where three different components, catechol-containing glycopolymer, HAuCl₄ , and amine-terminal CpG (CpG-NH₂ ) can react in a single step to generate target adjuvant. It is found that hetero-glycoadjuvant@AuNPs could increase adjuvant internalization and enhance the activation of bone-marrow-derived dendritic cells. Critically, lymphoseek-inspired vaccine potentiates antigen-specific CD8⁺ T-cell immune responses.