Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Social Foundations of Self I: Play and Game01:24

Social Foundations of Self I: Play and Game

195
The development of self in children is deeply rooted in social interactions, mainly through stages of play and structured games. These stages, outlined by sociologist George Herbert Mead, illustrate how children progressively learn to understand and adopt social roles, forming a cohesive sense of self.The Play Stage: Imitation and Simple Role-TakingIn the early years of childhood, the play stage is characterized by imitative behavior, where children engage in role-playing based on familiar...
195
The Tree of Life - Bacteria, Archaea, Eukaryotes02:40

The Tree of Life - Bacteria, Archaea, Eukaryotes

38.0K
The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
38.0K
Other Unique Bacteria01:18

Other Unique Bacteria

426
Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic...
426
The Roles of Bacteria and Fungi in Plant Nutrition02:11

The Roles of Bacteria and Fungi in Plant Nutrition

47.1K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
47.1K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

587
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
587
Hyperthermophilic Bacteria01:21

Hyperthermophilic Bacteria

508
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
508

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intracellular barriers and receptor masking limit success of a <i>Pseudomonas aeruginosa</i> clinical phage family.

bioRxiv : the preprint server for biology·2026
Same author

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
Same author

CBASS limits bacteriophage production while maintaining cell viability in Pseudomonas aeruginosa.

Cell host & microbe·2026
Same author

Balancing of immune activation and suppression during phage infection.

bioRxiv : the preprint server for biology·2026
Same author

CBASS limits bacteriophage production while maintaining cell viability in <i>Pseudomonas aeruginosa</i>.

bioRxiv : the preprint server for biology·2026
Same author

Translation-dependent degradation of cas12 mRNA triggered by an anti-CRISPR.

Nature·2026

Related Experiment Video

Updated: Jan 22, 2026

Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria
06:29

Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria

Published on: April 25, 2025

723

Cas13 Helps Bacteria Play Dead when the Enemy Strikes.

Senén D Mendoza1, Joseph Bondy-Denomy1

  • 1Department of Microbiology & Immunology, University of California, San Francisco, San Francisco, CA, 94143.

Cell Host & Microbe
|July 12, 2019
PubMed
Summary

The CRISPR-Cas13 system protects against viruses by detecting viral RNA and triggering cell dormancy, not by cutting viral DNA. This mechanism provides herd immunity against double-stranded DNA phages.

More Related Videos

Quantification of Proliferative and Dead Cells in Enteroids
10:47

Quantification of Proliferative and Dead Cells in Enteroids

Published on: January 31, 2020

8.9K
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
08:04

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Published on: February 8, 2019

9.1K

Related Experiment Videos

Last Updated: Jan 22, 2026

Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria
06:29

Live/Dead Staining for Quantifying Viable but Not Culturable Cells in Manuka Honey-Treated Wound-Causing Bacteria

Published on: April 25, 2025

723
Quantification of Proliferative and Dead Cells in Enteroids
10:47

Quantification of Proliferative and Dead Cells in Enteroids

Published on: January 31, 2020

8.9K
Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
08:04

Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining

Published on: February 8, 2019

9.1K

Area of Science:

  • Molecular Biology
  • Microbial Genetics
  • Bacteriophage Research

Background:

  • The precise mechanism of RNA-targeting CRISPR-Cas13 in phage defense remained unclear.
  • CRISPR-Cas systems are crucial for bacterial immunity against mobile genetic elements.

Purpose of the Study:

  • To elucidate the function of CRISPR-Cas13 as a phage defense system.
  • To investigate how Cas13 confers immunity against double-stranded DNA (dsDNA) phages.

Main Methods:

  • Observational studies on CRISPR-Cas13 activity in bacterial hosts.
  • Analysis of host-phage interactions and transcriptomic profiling.

Main Results:

  • Cas13 provides potent immunity against dsDNA phages without targeting their genomic DNA.
  • The system functions by sensing phage-derived RNA transcripts.
  • Nonspecific RNA degradation by Cas13 induces cellular dormancy, halting phage replication.

Conclusions:

  • CRISPR-Cas13 utilizes RNA sensing and non-specific RNA degradation to establish phage immunity.
  • This RNA-targeting mechanism confers 'herd immunity' by protecting the bacterial population from phage infection.