Related Experiment Video
Updated: Jan 22, 2026
09:46
Synthesis of Plant Phenol-derived Polymeric Dyes for Direct or Mordant-based Hair Dyeing
Published on: December 1, 2016
9.7K
Laccase isoform diversity in basidiomycete Lentinus strigosus 1566: Potential for phenylpropanoid polymerization
M P Kolomytseva1, N M Myasoedova1, A M Chernykh1
1G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of Russian Academy of Sciences (IBPM RAS), PSCBR RAS, Prospekt Nauki 5, Pushchino, Moscow Region 142290, Russia.
International Journal of Biological Macromolecules
|July 12, 2019
Summary
Researchers purified three laccase enzymes from Lentinus strigosus. Laccase III shows stability in neutral conditions, offering potential for biotechnological applications and creating novel pharmaceutical compounds.
Area of Science:
- Enzymology
- Biotechnology
- Mycology
Background:
- Basidiomycete Lentinus strigosus produces multiple laccase isoforms.
- Laccases are enzymes with diverse biotechnological applications, including polymer synthesis.
Purpose of the Study:
- To purify and characterize laccase isoforms from Lentinus strigosus.
- To evaluate the stability and activity of these laccases under various conditions.
- To explore their potential in synthesizing valuable pharmaceutical compounds.
Main Methods:
- Submerged cultivation of Lentinus strigosus.
- Purification of laccase isoforms using chromatography.
- Enzyme activity assays at different temperatures and pH.
- MALDI-TOF analysis for protein identification.
- Demonstration of phenylpropanoid transformation.
Main Results:
- Three laccase isoforms (Laccase I, II, III) with distinct properties were isolated.
- All isoforms exhibited optimal activity in acidic conditions around 70°C.
- Laccase III demonstrated significant stability and activity in neutral conditions for over a week.
- Activation phenomena were observed under specific temperature, pH, and sulfate conditions.
- Laccase III showed potential for biotechnological applications in neutral environments.
- Transformation of phenylpropanoids into polymers indicated potential for novel pharmaceutical analogue production.
Conclusions:
- Laccase III from Lentinus strigosus is a promising candidate for biotechnological applications due to its stability at neutral pH.
- The characterized laccases serve as a model for studying enzyme stability and activity regulation.
- Potential exists for producing valuable pharmaceutical compounds through enzymatic transformation of phenylpropanoids.
Related Concept Videos
01:30Diversity of Archaea I
566
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
566
01:24Diversity of Archaea II
465
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
465
01:15Diversity of Protists I
904
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
904
01:27Diversity of Protists II
842
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
842
01:13Cell Diversity
4.8K
The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular...
Multicellular...
4.8K
01:16Olefin Metathesis Polymerization: Ring-Opening Metathesis Polymerization (ROMP)
3.1K
Ring-opening metathesis polymerization or ROMP involves strained cycloalkenes as starting materials. The mechanism of ROMP proceeds by reacting cycloalkene with Grubbs catalyst to give metallacyclobutane intermediate which undergoes a ring-opening reaction to form new carbene. The new carbene reacts with another molecule of cycloalkene. Repetition of these steps leads to the formation of an unsaturated open-chain polymer product. All these steps are reversible, however, relieving the ring...
3.1K