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Updated: Jan 22, 2026

Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays
Published on: January 7, 2019
Comparing methods of genetic manipulation in Bacillus subtilis for expression of recombinant enzyme: Replicative or
Kamila Oliveira Santos1, João Costa-Filho1, Kérolin Luana Spagnol1
1Laboratory of Molecular Biology, Institute of Biological Sciences (ICB), Federal University of Rio Grande (FURG), Av. Itália, Km 8, 96203-900 Rio Grande, RS, Brazil.
Abstract:
The present study evaluated the stability of Bacillus subtilis strains transformed with a replicative or integrative plasmid (via CRISPR-Cas9) to express a recombinant phytase. Both transformation methods did not affect the growth of B. subtilis, but the stability of the construct and the enzymatic activity was reduced in the strain transformed with the replicative plasmid.
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