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Updated: Jan 22, 2026

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
Draft genome sequence of a clinical Acinetobacter baumannii isolate of new sequence type ST1688 from Saudi Arabia
Muhammad Yasir1, Muhammad Waseem Shah2, Asif A Jiman-Fatani3
1Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Objectives:
Acinetobacter baumannii has emerged as a prevalent multidrug-resistant nosocomial pathogen worldwide. Here we report the draft genome sequence of A. baumannii strain Ab174 isolated from a neonatal patient diagnosed with acute peritonitis.
Methods:
The draft genome sequence of A. baumannii Ab174 was determined using a MiSeq platform (Illumina Inc., San Diego, CA) using v.3, 2×30-bp chemistry. Genomic assembly was performed using SPAdes 3.9 algorithm.
Results:
The draft genome of A. baumannii Ab174 is 3 747 065bp in length and was classified as a new sequence type (ST1688). The genome of A. baumannii Ab174 has a G+C content of 39% and harbours two plasmids. The antimicrobial resistance gene blaADC-25 and the virulence factor gene for penicillin-binding protein G (pbpG) as well as 17 genomic islands and 14 insertion sequences were identified in the genome of A. baumannii Ab174.
Conclusion:
The genome sequence of A. baumannii strain Ab174 can be used as a reference sequence for the new ST1688. These data will facilitate further understanding of genomic variation in isolates from different geographical regions.
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