A novel impedance enhancer for amperometric biosensor based ultrasensitive detection of matrix metalloproteinase-2

The detection range and sensitivity is crucial to the determination of tumor markers. For amperometric biosensors, the detection range relies on the initial current signals (I₀) and the sensitivity is tightly related to the current signals difference (ΔI) caused by per unit concentration target. Herein, an amperometric biosensor was fabricated using polyaniline gel as substrate and CS-AuNPs-Pb²⁺ as impedance enhancer. The sensing substrate exhibited strong current signal in [Fe(CN)₆]^3-/4- due to the excellent conductivity and large specific surface area of polyaniline gel. CS-AuNPs-Pb²⁺ with large hindrance effect can significantly increase interfacial resistance, resulting in the enhancement of ΔI. In addition, Pb²⁺ can react with sodium tartrate to produce non-conducting sodium tartrate gel on sensing interface, leading to further amplification of ΔI. Matrix metalloproteinase-2 (MMP-2) was analysed to prove the feasibility of this strategy. The proposed amperometric biosensor reveal both wide linear detection range from 1 pg mL^-1 to 1 μg mL^-1 and high sensitivity of 28.4 μA·(LgC_MMP-2)^-1. Therefore, this strategy will be of great significance to design other ultrasensitive amperometric biosensors.