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Genome Sequences of Six Prophages Isolated from Staphylococcus pseudintermedius Strains Recovered from Human and
Juliette R K Wipf1, Douglas R Deutsch1, Lars F Westblade2,3
1Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York, USA.
Microbiology Resource Announcements
|July 13, 2019
Abstract:
Staphylococcus pseudintermedius is a common bacterial pathogen in companion animal medicine and has demonstrated zoonotic potential. Here, we report six new Staphylococcus pseudintermedius prophage genomes of the Siphoviridae family, identified in isolates recovered from human and canine clinical specimens.
Insights
Six new Staphylococcus pseudintermedius prophage genomes from the Siphoviridae family were identified. These bacterial viruses were found in clinical samples from both humans and dogs, highlighting potential zoonotic transmission routes.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Staphylococcus pseudintermedius is a significant bacterial pathogen in veterinary medicine.
- This bacterium possesses zoonotic potential, posing risks to human health.
- Bacteriophages, viruses that infect bacteria, play crucial roles in microbial ecology and evolution.
Purpose of the Study:
- To characterize novel prophage genomes from Staphylococcus pseudintermedius.
- To investigate the genetic diversity of Siphoviridae family phages associated with this pathogen.
- To explore the implications of these prophages in the context of S. pseudintermedius infections in companion animals and humans.
Main Methods:
- Whole-genome sequencing of bacterial isolates.
- Bioinformatic analysis for prophage genome identification and assembly.
- Phylogenetic analysis to determine phage relationships within the Siphoviridae family.
Main Results:
- Six complete prophage genomes belonging to the Siphoviridae family were identified.
- These prophages were found integrated within the genomes of S. pseudintermedius isolates.
- The identified prophages originated from clinical specimens of both canine and human origin.
Conclusions:
- The discovery of new S. pseudintermedius prophages expands our understanding of phage diversity.
- These findings provide insights into the genetic makeup of phages associated with a common veterinary pathogen.
- The presence of these prophages in both canine and human isolates warrants further investigation into their role in bacterial virulence and zoonotic potential.
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