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Updated: Jan 22, 2026

Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
Published on: November 5, 2020
Biomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles
Tomoya Horiuchi1, Reiji Masuda1, Hiroaki Murakami1
1Maizuru Fisheries Research Station, Field Science Education and Research Center, Kyoto University, Maizuru, Kyoto, Japan.
Abstract:
Environmental DNA (eDNA) from juvenile jack mackerel Trachurus japonicus was detected in tanks with 1, 3, 10, or 30 individuals per tank. Quantitative PCR using a set of species-specific primers and a probe revealed that the concentration of eDNA increased almost linearly with the density of fish. The coefficient of determination (R2 ) in the linear regression was lower than values previously reported for freshwater fishes in similar settings.
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