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Oncogene miR-934 promotes ovarian cancer cell proliferation and inhibits cell apoptosis through targeting BRMS1L

Y Hu1, Q Zhang, J Cui

  • 1Department of Gynecological Oncology, Shaanxi Provincial Tumor Hospital, Xi'an, China. gyhture001@163.com.

European Review for Medical and Pharmacological Sciences
|July 13, 2019
PubMed
Abstract

Insights

MicroRNA-934 (miR-934) promotes ovarian cancer cell growth and inhibits apoptosis by targeting BRMS1L. This suggests miR-934 and BRMS1L are potential therapeutic targets for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Ovarian cancer is a leading cause of cancer-related death in women.
  • MicroRNAs (miRNAs) are increasingly recognized as key regulators in ovarian cancer development and progression.

Purpose of the Study:

  • To investigate the role of microRNA-934 (miR-934) in ovarian cancer.
  • To identify the downstream target of miR-934 and its mechanism in regulating ovarian cancer cell behavior.

Main Methods:

  • Ovarian cancer cell lines (HO8910, OVCAR-3) were transfected with miR-934 inhibitor.
  • Cell proliferation and apoptosis were assessed using CCK-8 and TUNEL assays.
  • Gene and protein expression levels of related factors and BRMS1L were measured by qRT-PCR and Western blotting; target validation was performed using luciferase reporter assays.

Main Results:

  • miR-934 expression was elevated in ovarian cancer cells, while BRMS1L expression was decreased.
  • Inhibition of miR-934 suppressed cell proliferation and induced apoptosis, affecting Ki67, Cyclin D1, Caspase3, and Bcl-2 expression.
  • BRMS1L was confirmed as a direct target gene of miR-934.

Conclusions:

  • Oncogenic miR-934 promotes ovarian cancer cell proliferation and inhibits apoptosis by targeting BRMS1L.
  • miR-934 and BRMS1L represent potential novel biomarkers and therapeutic targets for ovarian cancer treatment.

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