Related Experiment Video

Updated: Jan 22, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.3K

MiR-299-3p inhibits proliferation and invasion of cervical cancer cell via targeting TCF4

Y Yu1, J-D Zhao, H Yang

  • 1Perinatal Care Division, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, P.R. China. yanghui_huaian@126.com.

European Review for Medical and Pharmacological Sciences
|July 13, 2019
PubMed
Abstract

Insights

MicroRNA-299-3p (miR-299-3p) functions as a tumor suppressor in cervical cancer (CC). Its reduced expression promotes CC cell growth and invasion by targeting transcription factor 4 (TCF4).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in human cancers, including cervical cancer (CC).
  • The specific role and regulatory mechanisms of miR-299-3p in CC progression are not well understood.

Purpose of the Study:

  • To investigate the expression profile of miR-299-3p in cervical cancer.
  • To elucidate the functional role of miR-299-3p in regulating CC cell proliferation and invasion.
  • To identify the molecular targets of miR-299-3p in cervical cancer.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) for miR-299-3p expression analysis.
  • Cell proliferation, colony formation, and Transwell invasion assays to assess cellular functions.
  • Luciferase reporter and Western blot assays to validate direct target interactions.

Main Results:

  • miR-299-3p expression was significantly downregulated in CC cell lines.
  • Overexpression of miR-299-3p suppressed cervical cancer cell proliferation and invasion.
  • Transcription factor 4 (TCF4) was identified as a direct downstream target of miR-299-3p.
  • TCF4 restoration counteracted the inhibitory effects of miR-299-3p in CC cells.

Conclusions:

  • miR-299-3p acts as a tumor suppressor in cervical cancer.
  • The miR-299-3p/TCF4 axis plays a critical role in regulating CC cell behavior.
  • Targeting miR-299-3p may offer a therapeutic strategy for cervical cancer.

More Related Videos

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.4K
Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish09:22

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Published on: April 25, 2017

17.4K

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.7K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
56.9K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.0K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
91.7K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.6K