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Updated: Jan 22, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
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Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

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Long noncoding RNA DDX11-AS1 induced by YY1 accelerates colorectal cancer progression through targeting miR-873/CLDN7

J-B Tian1, L Cao, G-L Dong

  • 1Department of General Surgery, the Frist Medical Centre of Chinese of General Hospital, Haidian, Beijing, China. tianjingbo1226@126.com.

European Review for Medical and Pharmacological Sciences
|July 13, 2019
PubMed
Abstract

Insights

Long non-coding RNA DDX11-AS1 is upregulated in colorectal cancer (CRC), promoting tumor growth and metastasis. Targeting DDX11-AS1 may offer a new therapeutic strategy for CRC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators in cancer development.
  • LncRNA DDX11 antisense RNA 1 (DDX11-AS1) has shown aberrant expression in various tumors, prompting investigation in colorectal cancer (CRC).

Purpose of the Study:

  • To investigate the expression patterns and functional roles of lncRNA DDX11-AS1 in colorectal cancer (CRC).
  • To explore DDX11-AS1 as a potential diagnostic and therapeutic target for CRC.

Main Methods:

  • Utilized The Cancer Genome Atlas (TCGA) datasets for lncRNA identification in CRC.
  • Quantified DDX11-AS1 expression in CRC tissues and cell lines using Real Time-Polymerase Chain Reaction (RT-PCR).
  • Performed clinical significance analysis (Chi-square, Kaplan-Meier), functional assays, ChIP, and luciferase reporter assays to elucidate mechanisms.

Main Results:

  • DDX11-AS1 was significantly upregulated in CRC specimens and cell lines, partly induced by YY1.
  • Elevated DDX11-AS1 expression correlated with lymph node metastasis, advanced TNM stage, and poorer overall survival in CRC patients.
  • Knockdown of DDX11-AS1 inhibited CRC cell proliferation, migration, and invasion, while promoting apoptosis.
  • Mechanistically, DDX11-AS1 sponges miR-873, preventing CLDN7 degradation and facilitating CRC progression.

Conclusions:

  • DDX11-AS1 is a novel, independent prognostic biomarker for colorectal cancer.
  • The findings highlight DDX11-AS1 as a potential therapeutic target for improving CRC treatment outcomes.

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