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MicroRNA 34b inhibits cell proliferation in pediatric acute myeloid leukemia via regulating LDHA

H-X Qi1, Q Cao, G-P Zhou

  • 1Department of Pediatrics, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huaian, China. kwx9743@163.com.

European Review for Medical and Pharmacological Sciences
|July 13, 2019
PubMed
Abstract

Insights

MicroRNA-34b is downregulated in pediatric acute myeloid leukemia (AML), indicating poor prognosis. This microRNA regulates leukemia cell proliferation and apoptosis by targeting lactate dehydrogenase A (LDHA).

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Pediatric acute myeloid leukemia (AML) is a significant health concern.
  • Understanding the molecular mechanisms underlying AML is crucial for improving patient outcomes.

Purpose of the Study:

  • To investigate the role of microRNA-34b in pediatric AML.
  • To elucidate the regulatory effect of microRNA-34b on leukemia cell behavior and its underlying mechanism involving lactate dehydrogenase A (LDHA).

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to detect microRNA-34b expression in pediatric AML patients.
  • Cell counting kit-8 (CCK-8) assay and flow cytometry to assess proliferation, cell cycle, and apoptosis.
  • Bioinformatics prediction and dual-luciferase reporter gene assay to confirm the interaction between microRNA-34b and LDHA.
  • Rescue experiments to validate the regulatory pathway.

Main Results:

  • MicroRNA-34b expression was significantly downregulated in children with AML.
  • Low microRNA-34b expression correlated with specific FAB typing, cytogenetic abnormalities, and poor treatment response.
  • Reduced microRNA-34b levels were associated with a poorer prognosis in pediatric AML.
  • Overexpression of microRNA-34b inhibited leukemia cell proliferation and cell cycle progression while promoting apoptosis.
  • MicroRNA-34b directly targets and inhibits LDHA expression, and LDHA overexpression can reverse the effects of microRNA-34b on leukemia cells.

Conclusions:

  • MicroRNA-34b is downregulated in pediatric AML and may serve as a prognostic biomarker.
  • MicroRNA-34b influences leukemia cell proliferation and apoptosis by regulating LDHA expression.

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