Related Experiment Video
Updated: Jan 22, 2026

Demonstration of the Rat Ischemic Skin Wound Model
Published on: April 1, 2015
Wound Healing and Antimicrobial Effects of Chitosan-hydrogel/Honey Compounds in a Rat Full-thickness Wound Model
Jebrail Movaffagh1, Bibi Seddigheh Fazly Bazzaz1, Abbas Tabatabaei Yazdi2
1Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Introduction:
Honey and chitosan have shown antimicrobial and wound healing effects. As a biocompatible and biodegradable biomaterial, chitosan has shown antimicrobial capabilities.
Objective:
In this study, the effects of the incorporation of high molecular weight chitosan hydrogel on antibacterial, antifungal, and wound healing properties of honey were investigated.
Materials And Methods:
The minimum inhibitory concentration of chitosan and honey were examined in pure and 3:1, 1:1, and 1:3 (v/v) compound ratios for Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In addition, the inflammatory, granulation and fibrotic tissue formation, reepithelialization indices, and wound shrinkage effects of each treatment were evaluated and compared with saline and silver sulfadiazine.
Results:
Chitosan/honey 1:3 was found to be effective against all 5 aforementioned germs. Honey, chitosan/honey 1:1, and chitosan/honey 1:3 showed faster wound healing and shrinkage effects.
Conclusions:
Incorporation of chitosan hydrogel into honey can substantially enhance its antimicrobial and wound healing effects. Chitosan-hydrogel/honey (1:3) is an optimal wound dressing formulation with ample antimicrobial and healing properties.
Related Concept Videos
Antimicrobial Effectiveness
Phases of Wound Repair
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
π Electron Effects on Chemical Shift: Aromatic and Antiaromatic Compounds
Antimicrobial Proteins
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Molecules and Compounds
Organic Compounds

