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4'-Guanidinium-modified siRNA: a molecular tool to control RNAi activity through RISC priming and selective antisense
Ganesh N Nawale1, Saeed Bahadorikhalili, Pallabi Sengupta
1Translational Chemical Biology Laboratory, Division of Polymer Chemistry, Department of Chemistry-Ångström, Uppsala University, Uppsala, Sweden. oommen.varghese@kemi.uu.se.
Chemical Communications (Cambridge, England)
|July 13, 2019
Abstract:
We designed novel 4'-C-guanidinocarbohydrazidomethyl-5-methyl uridine (GMU) modified small interfering RNA (siRNA) and evaluated its biophysical and biochemical properties. Incorporation of GMU units significantly increased the thermodynamic stability as well as the enzymatic stability against nucleases in human serum. A gene silencing experiment indicated that GMU modfied siRNA (siRNA6) resulted in ≈4.9-fold more efficient knockdown than unmodified siRNA.