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Updated: Jan 22, 2026

Quantitative and Qualitative Examination of Particle-particle Interactions Using Colloidal Probe Nanoscopy
Published on: July 18, 2014
Sub-micron sized cytochrome c particles adsorbing to solid surfaces: A comparison between solution phase and
Maheshkumar Jaganathan1, S Easwaramoorthy2, A Dhathathreyan3
1CATERS, CSIR-CLRI, Adyar, Chennai 600020, India.
Abstract:
Present work reports on Cytochrome C (Cyt C) adsorption from solution and as sub-micron sized colloidal particles (pH 7.5) at fluid/solid interface. The colloidal particles from 2 methods ((method 1 freeze-thaw); (method 2-Decane/water interface)) have been characterized by UV-Visible spectroscopy, Static and Time resolved Fluorescence spectra and Circular dichroic spectroscopy. Morphology and size studies indicate that the colloids are solid and well-packed. Using Quartz crystal microbalance with dissipation, elastic compliance of the protein on quartz surface has been monitored. Properties of the protein from solution and as colloids assembled from method 2 are similar in elastic compliance (65.53 ± 1.2 and 73.5 ± 1.1 GPa-1 respectively) due to polar/non-polar interactions at the solid surface. For particles from method 1, irregular desolvation of water on the particle surface results in higher compliance (104.3 ± 1.3 GPa-1). Change in work of adhesion from contact angle profiles shows optimal adhesion for colloids from method 1 whereas the protein solution and as colloids from method 2, show subnormal adhesion. The work shows the role of extensive H-bonding with the hydrodynamically coupled water leading to a fluid like system in protein colloids from method 1 whereas those from method 2 behaved more like the pure protein adsorbing from solution.
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