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Genotypic identification of Pseudomonas aeruginosa strains isolated from patients with urinary tract infection
Mohammad Estaji1, Mohsen Tabasi2, Fatemah Sadeghpour Heravi3
1Molecular Medicine Department, Medical Institute, National Institute of Genetics and Biotechnology, Tehran, Iran.
Background:
Numerous nosocomial infections including urinary tract infection (UTI) have been reported to be linked to Pseudomonas aeruginosa (P. aeruginosa). This bacterium is one of the most common pathogen colonized in the urinary tract. The main purpose of this study was to evaluated the presence of antibiotic resistance genes and also the most frequent genotype patterns of P. aeruginosa in the patients with UTI hospitalized in different wards of hospitals.
Materials And Methods:
In this study, 70 strains of P. aeruginosa isolated of urine samples from the patients with UTI were assessed. The isolated strains were genotyped using Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) method. We have also analyzed the presence of TEM and SHV resistant genes in the isolates.
Results:
A total of 70 P. aeruginosa strains was isolated from the UTI patients. Based on MLVA method, 61 various genotypes of P. aeruginosa were identified which grouped into two main clusters and 4 sub-clusters. Moreover, approximately 80% and 70% of isolated strains carried the TEM and SHV resistance genes, respectively.
Conclusion:
Our findings showed that the majority of patients hospitalized in different wards of hospitals have experienced the urinary tract infection caused by P. aeruginosa. According to the genotyping results, a high diversity of the P. aeruginosa population was observed in the patients with UTI. Our results can provide a better understanding of the P. aeruginosa genotype distribution and epidemiology of infection, which can be applied as basic data for future antibiotic therapies.
Insights
Pseudomonas aeruginosa causes many hospital-acquired urinary tract infections (UTIs). This study found a high diversity of P. aeruginosa genotypes and common antibiotic resistance genes (TEM and SHV) in UTI patients, informing future treatments.
Area of Science:
- Medical Microbiology
- Infectious Diseases
- Genetics
Background:
- Pseudomonas aeruginosa (P. aeruginosa) is a common cause of nosocomial urinary tract infections (UTIs).
- Understanding P. aeruginosa epidemiology and resistance is crucial for infection control.
Purpose of the Study:
- To evaluate antibiotic resistance genes (TEM, SHV) in P. aeruginosa from UTI patients.
- To determine the prevalent genotype patterns of P. aeruginosa in hospitalized UTI patients.
Main Methods:
- Analyzed 70 P. aeruginosa strains isolated from urine samples of UTI patients.
- Utilized Multiple-Locus Variable Number Tandem Repeat Analysis (MLVA) for genotyping.
- Assessed the presence of TEM and SHV resistance genes.
Main Results:
- Identified 61 distinct P. aeruginosa genotypes among the 70 isolates using MLVA.
- These genotypes clustered into two main and four sub-clusters, indicating high diversity.
- Approximately 80% and 70% of strains carried TEM and SHV resistance genes, respectively.
Conclusions:
- Hospitalized patients frequently experience UTIs caused by P. aeruginosa.
- Genotyping revealed significant diversity in P. aeruginosa populations within UTI patients.
- Findings provide essential data for understanding P. aeruginosa epidemiology and guiding future antibiotic therapies.
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