Multi-modal image cytometry approach - From dynamic to whole organ imaging

Area of Science:

• Life Sciences
• Biotechnology
• Microscopy

Background:

• Optical imaging visualizes biological processes within tissues.
• Different modalities offer distinct information: time-lapse for dynamics, light sheet for whole tissues, and confocal for high resolution.
• Multi-modal imaging and image cytometry enable quantitative, objective comparisons between sample groups.

Purpose of the Study:

• To provide an overview of technical considerations for image cytometry workflows.
• To discuss sample preparation, post-processing, and analysis for intra-vital and whole organ imaging.

Main Methods:

• Exploration of time-lapse, light sheet, and confocal microscopy techniques.
• Integration of multi-modal imaging approaches.
• Application of image cytometry for quantitative data analysis.

Main Results:

• Detailed technical aspects of an image cytometry workflow are presented.
• Challenges and solutions for sample preparation are discussed.
• Guidance on image post-processing and analysis for specific imaging types is provided.

Conclusions:

• Image cytometry, combined with advanced optical imaging, offers robust quantitative analysis for biological studies.
• Effective workflow design, including sample preparation and data analysis, is crucial for reliable results.
• This overview aids researchers in implementing image cytometry for intra-vital and whole organ investigations.