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Updated: Jan 22, 2026

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
Published on: May 9, 2016
Displacement fields using correlation methods as a tool to investigate cell migration in 3D collagen gels
Arnold Fertin1, Laure Laforgue2,3, Alain Duperray2
1CNRS, TIMC-IMAG, University Grenoble Alpes, Grenoble, France.
Abstract:
Living cells embedded in a complex extra-cellular matrix migrate in a sophisticated way thanks to adhesions to matrix fibres and contractility. It is important to know what kind of forces are exerted by the cells. Here, we use reflectance confocal microscopy to locate fibres accurately and determine displacement fields. Correlation techniques are used to this aim, coupled with proper digital image processing. Benchmark tests validate the method in the case of shear and stretching motions. Finally, the method is tested successfully for studying cancer cells migrating in collagen gels of different concentration.
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