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Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
Published on: February 24, 2017
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Pseudo-bipolar spindle formation and cell division in postnatal binucleated cardiomyocytes
1Experimental Renal and Cardiovascular Research, Department of Nephropathology, Institute of Pathology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Schwabachanlage 12, 91054 Erlangen, Germany.
Journal of Molecular and Cellular Cardiology
|July 14, 2019
Summary
Postnatal binucleated cardiomyocytes can divide after stimulation. These cells manage multiple centrioles by forming pseudo-bipolar spindles, enabling cell division or cytokinesis failure.
Area of Science:
- Cardiology
- Cell Biology
- Regenerative Medicine
Background:
- Most adult cardiomyocytes are not diploid mononucleated, yet heart regeneration research focuses on diploid cells.
- Binucleated cardiomyocytes are abundant but understudied in terms of proliferation and cell division.
- This study investigates mitosis and cell division in postnatal binucleated cardiomyocytes.
Purpose of the Study:
- To understand the process of mitosis and cell division in postnatal binucleated cardiomyocytes.
- To investigate the behavior of binucleated cardiomyocytes when stimulated to re-enter the cell cycle.
Main Methods:
- Stimulation of postnatal rat binucleated cardiomyocytes with fetal bovine serum or FGF1/p38 inhibitor.
- Phase-contrast video microscopy to observe cell division.
- Immunofluorescence for centriole number analysis.
- Fluorescence live imaging of tubulin-GFP to observe spindle formation.
Main Results:
- Binucleated cardiomyocytes form a single metaphase plate and often fail cytokinesis.
- A maximum of 6% cell division rate was observed in video-recorded binucleated cardiomyocytes.
- Mitotic binucleated cardiomyocytes possess >4 centrioles, leading to multipolar spindle formation that resolves into pseudo-bipolar spindles.
- Centriole distribution to daughter cells was frequently uneven.
Conclusions:
- Postnatal binucleated cardiomyocytes can enter mitosis upon stimulation.
- These cells adapt to multiple/unpaired centrioles by forming pseudo-bipolar spindles.
- The study demonstrates that binucleated cardiomyocytes can divide, despite challenges with centriole number and spindle organization.
Keywords:
Binucleated cardiomyocyteCell divisionCentrioleCentrosomeIntermediate multipolesLive cell imagingMore Related Videos
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