Characterization of a replication-competent vector encoding DsRed based on a human adenovirus type 4 a-like strain

Area of Science:

• Virology
• Molecular Biology
• Immunology

Background:

• Human adenovirus type 4 (HAdV4) causes acute respiratory disease (ARD) in various age groups.
• HAdV4 strains are classified into prototype (p)-like and a-like genomic clusters.

Purpose of the Study:

• To report the complete genome sequence of a novel HAdV4 strain (GZ01) from China.
• To generate and characterize a replication-competent recombinant HAdV4 (rAd4DsRed) for potential applications.
• To establish a rapid method for detecting neutralizing antibodies against HAdV3 and HAdV4.

Main Methods:

• Whole-genome sequencing and in silico analysis of HAdV4 GZ01.
• Construction and characterization of a replication-competent recombinant adenovirus expressing DsRed.
• Infection assays in cell lines and animal models.
• Immunization studies in mice.
• Development of a multiplex assay for antibody detection.

Main Results:

• The HAdV4 GZ01 strain was identified as genome-type 4a1.
• Recombinant rAd4DsRed efficiently infected cells and expressed DsRed, with no alterations in antigenicity or replication compared to wild-type HAdV4.
• Mice immunized with rAd4DsRed showed a significant antibody response to DsRed.
• A rapid assay for detecting neutralizing antibodies against HAdV3 and HAdV4 was successfully established.

Conclusions:

• The HAdV4 GZ01 strain and the rAd4DsRed vector provide a basis for developing HAdV4 vaccines.
• The recombinant adenovirus platform holds promise for vaccine and gene therapy applications.
• The developed assay enables rapid serological and antiviral screening.