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Related Experiment Video

Updated: Jan 22, 2026

A Method for Characterizing Embryogenesis in Arabidopsis

A Method for Characterizing Embryogenesis in Arabidopsis

Published on: August 4, 2017

Hectd1 is essential for embryogenesis in mice.

Donato D'Alonzo¹, Fabienne Hélène Emch², Xiaoli Shen³

¹Department of Biomedicine, University Hospital, University of Basel, Hebelstrasse 20, CH-4031, Basel, Switzerland.

Gene Expression Patterns : GEP

|July 14, 2019

Summary

The E3 ubiquitin ligase Hectd1 is essential for embryonic development, with mutations causing early lethality and placental defects. This study introduces a new Hectd1 mutant mouse model for further research.

Keywords:

Gene expression Gene trap Hectd1 Mutant mouse X-gal

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Area of Science:

Molecular Biology
Developmental Biology
Genetics

Background:

The functional roles of the E3 ubiquitin ligase Hectd1 in embryogenesis and cell biology require further investigation.
Understanding Hectd1's function is crucial for comprehending developmental processes and potential cellular pathologies.

Purpose of the Study:

To generate and characterize a novel transgenic mouse model for Hectd1 to study its biological functions.
To investigate the consequences of Hectd1 deficiency during embryonic development.

Main Methods:

Generation of a Hectd1 mutant mouse model using the gene trap strategy with a β-geo cassette insertion.
Analysis of embryonic lethality, placental development, and neural tube closure in homozygous and heterozygous Hectd1-mutant mice.
X-gal staining to determine the temporal and spatial expression patterns of Hectd1 in adult tissues.

Main Results:

Homozygous Hectd1-mutant mice exhibited early embryonic lethality, abnormal placental development (thinner placentas, particularly in the labyrinth layer), and neural tube closure defects (exencephaly).
Hectd1 expression was observed in specific cell populations across various adult tissues.
Hectd1 expression was found to be regulated by insulin, heat, and hypoxia.

Conclusions:

Hectd1 is indispensable for normal embryogenesis and fetal survival.
The generated Hectd1 mutant mouse model offers a valuable tool for detailed functional studies of Hectd1 in mammalian cells.