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Circulating cell-free DNA fragment analysis by microchip electrophoresis and its relationship with DNase I in cardiac

Junko Fujihara1, Yoshikazu Takinami2, Misuzu Ueki3

  • 1Department of Legal Medicine, Faculty of Medicine, Shimane University, 89-1 Enya, Izumo, Shimane 693-8501, Japan.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|July 15, 2019
PubMed

Insights

Circulating cell-free DNA (cfDNA) levels are elevated in cardiac patients. A specific cfDNA fragment ratio shows promise as a novel diagnostic indicator for myocardial infarction (MI).

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiology

Background:

  • Circulating cell-free DNA (cfDNA) is linked to various diseases, including cardiovascular conditions.
  • Understanding cfDNA characteristics in cardiac patients is crucial for diagnostic advancements.

Purpose of the Study:

  • To investigate plasma cfDNA concentrations and fragment distributions in cardiac disease patients.
  • To explore the relationship between cfDNA levels, deoxyribonuclease I (DNase I) activity, and myocardial infarction (MI).

Main Methods:

  • Plasma cfDNA was extracted from cardiac patients and healthy controls.
  • cfDNA fragment analysis was performed using microchip electrophoresis.
  • Deoxyribonuclease I (DNase I) activity was measured and correlated with cfDNA concentration.

Main Results:

  • Significantly higher cfDNA concentrations were observed in MI and cardiac angina patients compared to controls.
  • Cardiac patients exhibited three cfDNA fragments (150-200 bp, 300-400 bp, 500-600 bp), while controls showed a single fragment.
  • A 150-200 bp/500-600 bp cfDNA ratio was more prevalent in MI patients, and a positive correlation between DNase I activity and cfDNA concentration was found.

Conclusions:

  • Plasma cfDNA in cardiac disease patients may originate from apoptosis.
  • The 150-200 bp/500-600 bp cfDNA fragment ratio could serve as a novel diagnostic biomarker for MI.
  • DNase I activity is positively correlated with cfDNA concentration in cardiac patients.

Keywords:
ApoptosisCardiac diseaseDNase IMicrochip electrophoresiscfDNA

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