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Updated: Jan 22, 2026

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes
Published on: March 2, 2020
Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux
M Rhia L Stone1, Muriel Masi2, Wanida Phetsang1
1Institute for Molecular Bioscience , The University of Queensland , Brisbane , QLD 4072 , Australia .
Abstract:
Fluorescent probes derived from the fluoroquinolone antibiotic ciprofloxacin were synthesised using a Cu(i)-catalysed azide-alkyne cycloaddition (CuAAC) to link a ciprofloxacin azide derivative with alkyne-substituted green and blue fluorophores. The azide (2) and fluorophore (3 and 4) derivatives retained antimicrobial activity against Gram-positive and Gram-negative bacteria. The use of confocal fluorescent microscopy showed intracellular penetration, which was substantially enhanced in the presence of carbonyl cyanide 3-chlorophenylhydrazone as an efflux pump inhibitor in Escherichia coli.
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