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Direct interaction of food derived colloidal micro/nano-particles with oral macrophages

Lijing Ke1, Huiqin Wang1, Guanzhen Gao1

  • 1Food Nutrition Sciences Centre, Zhejiang Gongshang University, Hangzhou, China.

NPJ Science of Food
|July 16, 2019
PubMed

Insights

Food particles from bone soup interact with oral immune cells. These micro/nano-particles (MNPs) are internalized by macrophages, influencing their response to oxidative stress.

Area of Science:

  • Food science
  • Immunology
  • Nanotechnology

Background:

  • Bone soup contains colloidal micro/nano-particles (MNPs) that contact immune cells upon ingestion.
  • The interaction between food MNPs and oral immune cells is not well understood.

Purpose of the Study:

  • To investigate the uptake of bone soup MNPs by murine oral macrophages.
  • To determine the effects of MNP uptake on macrophage function.

Main Methods:

  • MNPs and NPs were isolated from porcine bone soup using ultrafiltration (UF) and size-exclusion chromatography (SEC).
  • Particles were labeled with Nile Red and co-incubated with murine oral macrophages.
  • Cellular responses were assessed using fluorescent dyes DiBAC4(3) and MitoSOX Red, and by challenging macrophages with AAPH.

Main Results:

  • Bone soup particles (UF-MNPs and SEC-NPs) were internalized by oral macrophages.
  • MNP uptake did not affect normal macrophage membrane potential or mitochondrial superoxide levels.
  • Engulfment of MNPs mitigated peroxyl radical-induced membrane hyperpolarization and suppressed mitochondrial respiration in challenged macrophages.

Conclusions:

  • Food colloidal particles directly interact with oral immune cells.
  • This interaction may represent a novel pathway for food-body communication.
Keywords:
Mucosal immunologyNanoparticles

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