Related Experiment Video
Updated: Jan 22, 2026

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
Published on: March 6, 2018
Detection of a prostate cancer cell line using a bioluminescent affiprobe: An attempt to develop a new molecular
Zahra Ghafouri Varnosfaderani1, Rahman Emamzadeh1, Mahboobeh Nazari2
1Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
Abstract:
Molecular probes have become an important tool to decipher cell and molecular events, as well as contributing to clinical diagnosis. In this study, we report on a novel bioluminescence affiprobe and evaluate its functionality by monitoring HER2 expression in the prostate cancer cells. The proposed bioluminescence affiprobe consists of a binding domain, an HER2 specific affibody molecule and a bioluminescent domain, recombinant Renilla luciferase. The experiments indicate that bioluminescence affiprobe can serve as a reliable and user-friendly probe for the detection of human HER2 positive prostate cells as well as ex vivo based detection of HER2-positive human prostate cancer specimens using luminometeric-based assays.
Related Concept Videos
Mouse Models of Cancer Study
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Molecular Factors Affecting Cell Division
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
Molecular Models
Molecular Orbital Theory II
Molecular and Ionic Solids
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...

