Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chemical Reactions in Aqueous Solutions

Chemical Reactions in Aqueous Solutions

Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.

Aqueous Solutions and Heats of Hydration

Aqueous Solutions and Heats of Hydration

Water and other polar molecules are attracted to ions. The electrostatic attraction between an ion and a molecule with a dipole is called an ion-dipole attraction. These attractions play an important role in the dissolution of ionic compounds in water.
When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...

Leveling Effect and Non-Aqueous Acid-Base Solutions

Leveling Effect and Non-Aqueous Acid-Base Solutions

This lesson defines the leveling effect in acidic and basic solutions and its role in aqueous and non-aqueous solutions. It is essential to understand the competing nature of various species in a chemical system.
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):

Range

Range

The range is one of the measures of variation. It can be defined as the difference between a dataset's highest and lowest values. For example, in the study of seven 16-ounce soda cans, the filled volume of soda was measured, thus producing the following amount (in ounces) of soda:
15.9; 16.1; 15.2; 14.8; 15.8; 15.9; 16.0; 15.5
Measurements of the amount of soda in a 16-ounce can vary since different subjects record these measurements or since the exact amount - 16 ounces of liquid, was not...

General Properties of Solutions

General Properties of Solutions

Many common substances around us exist as a solution, such as ocean water, air, and gasoline. All solutions are mixtures of substances that are composed of varying amounts of two or more types of atoms or molecules. A mixture with a non-uniform composition is a heterogeneous mixture, whereas a mixture with a uniform composition is a homogeneous mixture. The components that make the homogeneous mixture are evenly spread out and thoroughly mixed.

Solution Formation

Solution Formation

There is no one solvent that can dissolve every type of solute. Some substances that readily dissolve in a certain solvent might be insoluble in a different solvent. A simple way to predict which substances dissolve in which solvent is the phrase "like dissolves like". This means that polar substances, such as salt and sugar, dissolve in a polar substance like water. In contrast, non-polar substances are more soluble in non-polar solvents such as carbon tetrachloride.
This selective...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Structural, digestive, and physicochemical properties of heat-moisture treated potato starch complexed with peptides derived from Huizhou ham trimmings.

International journal of biological macromolecules·2026

Same author

Unraveling taste enhancement mechanism of yeast-inoculated reduced-salt fermented sausage: Proteolysis, taste compound formation, and receptor binding of potential salty/salty taste-enhancing peptides.

Food microbiology·2026

Same author

Identification and Characterization of Novel Anti-inflammatory and Hepatoprotective Properties of Dual-Function Peptides Derived from Jinhua Ham: A Study Integrating Computational Modeling with the cGAS-STING Pathway.

Journal of agricultural and food chemistry·2026

Same author

Chitosan-coated zein microcapsules for rumen-protected docosahexaenoic acid delivery and enrichment in goat milk.

International journal of biological macromolecules·2026

Same author

Sodium carboxymethylcellulose-sodium caseinate complex hydrocolloid enhances the oil barrier properties of batter-coated deep-fried pork.

Journal of the science of food and agriculture·2026

Same author

Myofibrillar Fragmentation and Cytoskeletal Protein Degradation: A Mechanism Driven by Ultra-High Pressure Processing to Tenderize Post-Mortem Goose Meat.

Journal of texture studies·2026

Search research articles

Related Experiment Video

Updated: Jan 22, 2026

Design and Characterization Methodology for Efficient Wide Range Tunable MEMS Filters

Design and Characterization Methodology for Efficient Wide Range Tunable MEMS Filters

Published on: February 4, 2018

A dansyl fluorescent pH probe with wide responsive range in aqueous solution.

Yan Wang¹, Lihan Zeng¹, Juntan Zhou¹

¹School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, PR China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|July 16, 2019

Summary

A novel fluorescent pH probe, FF-Dns, demonstrates sensitive detection across a broad pH range (1-13). This diphenylalanine-modified dansyl probe exhibits distinct color changes, indicating its potential for physiological pH monitoring.

Keywords:

Dansyl group Fluorescence probe Wide pH range pH sensitivity

More Related Videos

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Published on: September 7, 2018

Related Experiment Videos

Last Updated: Jan 22, 2026

Design and Characterization Methodology for Efficient Wide Range Tunable MEMS Filters

Design and Characterization Methodology for Efficient Wide Range Tunable MEMS Filters

Published on: February 4, 2018

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Visualization of Bacterial Resistance using Fluorescent Antibiotic Probes

Published on: March 2, 2020

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Generation and Control of Electrohydrodynamic Flows in Aqueous Electrolyte Solutions

Published on: September 7, 2018

Area of Science:

Chemical Sensors
Fluorescent Probes
Analytical Chemistry

Background:

Accurate pH monitoring is crucial in biological and chemical systems.
Development of sensitive and wide-range pH probes remains an active research area.

Purpose of the Study:

To design and synthesize a novel fluorescent pH probe based on diphenylalanine-modified dansyl (FF-Dns).
To evaluate the pH-sensing capabilities of FF-Dns across a wide pH range.

Main Methods:

Synthesis of the diphenylalanine-modified dansyl (FF-Dns) probe.
Spectroscopic analysis of FF-Dns fluorescence emission in aqueous solutions at varying pH levels (1-13).

Main Results:

FF-Dns exhibited sensitive fluorescence response to pH changes from 1 to 13.
Characteristic yellow fluorescence was observed between pH 4-10.
A blue-shift to green emission occurred at pH 11-13 due to sulfonamide deprotonation.
Very weak fluorescence was noted at pH 1-4 due to dimethylamino group protonation.

Conclusions:

The FF-Dns probe displays distinct fluorescence properties across a wide pH spectrum.
These pH-dependent spectral changes suggest FF-Dns is a promising candidate for detecting pH variations in physiological environments.