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A dansyl fluorescent pH probe with wide responsive range in aqueous solution
Yan Wang1, Lihan Zeng1, Juntan Zhou1
1School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, PR China.
Abstract:
A fluorescent pH probe based on diphenylalanine-modified dansyl (FF-Dns) was designed and synthesized. The probe showed sensitive fluorescence emission to pH change over a wide range of 1-13 in aqueous solution. At pH 4-10, FF-Dns displayed the characteristic fluorescent emission of dansyl group (yellow); while protonation of the dimethylamino group caused a very weak fluorescence emission at pH 1-4, and deprotonation of the sulfonamide group caused an emission blue-shift to the green region at pH 11-13. These properties endow FF-Dns with the potential to detect pH variation in physiological environments.
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