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Updated: Jan 22, 2026

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer
Published on: July 25, 2025
A new ratiometric fluorescent probe for sensing lysosomal HOCl based on fluorescence resonance energy transfer
He Meng1, Xiao-Qing Huang1, Yu Lin1
1School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University & Shandong Academy of Medical Sciences, Taian 271016, PR China.
Abstract:
In this paper, ratiometric imaging of lysosomal HOCl was realized with a molecular probe (CR-Ly) based on fluorescence resonance energy transfer by using coumarin as the donor and rhodamine as acceptor. CR-Ly showed high sensitivity and fast response to HOCl. Moreover, CR-Ly exhibited excellent selectivity and sensitivity for HOCl over other biologically relevant species. Furthermore, it was successfully utilized to image the endogenous HOCl with low cytotoxity. And CR-Ly was capable of targeting lysosomes and monitoring lysosomal hypochlorous acid changes owing to the presence of the morpholine moiety. We believe that probe CR-Ly would be helpful to further research on the HOCl-associated diseases in lysosomes.
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