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Investigating the ability of methicillin-resistant Staphylococcus pseudintermedius isolates from different sources to

Nathita Phumthanakorn1, Nuvee Prapasarakul1

  • 1Department of Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan, Bangkok 10330, Thailand (Phumthanakorn, Prapasarakul); Diagnosis and Monitoring of Animal Pathogens Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Road, Pathumwan, Bangkok 10330, Thailand (Prapasarakul).

Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire
|July 17, 2019
PubMed

Insights

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) adherence to skin cells was studied. While MRSP ST45 showed the highest adherence, cell wall protein genes did not predict this ability.

Area of Science:

  • Veterinary Microbiology
  • Dermatology
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is an opportunistic pathogen causing infections in animals and humans.
  • Understanding MRSP adherence to host cells is crucial for developing effective treatment and prevention strategies.
  • Cell wall-associated (CWA) proteins play a role in bacterial adhesion and colonization.

Purpose of the Study:

  • To investigate the adherence capability of different MRSP isolates to canine and human corneocytes.
  • To examine the relationship between cell wall-associated protein gene profiles and MRSP adherence.
  • To identify potential virulence factors associated with MRSP adhesion.

Main Methods:

  • Five MRSP isolates from various sources (canine, human, environmental) were used.
  • Adherence assays were performed using canine and human corneocytes.
  • Cell wall-associated (CWA) protein gene profiles of the MRSP isolates were analyzed.

Main Results:

  • MRSP isolates belonging to sequence type (ST) 45 exhibited the highest adherence to both canine and human corneocytes.
  • The number of CWA protein genes varied among the MRSP strains, ranging from 12 to 18.
  • Despite variations in CWA protein gene profiles, adherence ability could not be reliably predicted from these profiles.

Conclusions:

  • MRSP ST45, a prevalent clone in Thailand, demonstrates significant adherence to host epithelial cells.
  • The profile of cell wall-associated protein genes does not serve as a consistent predictor of MRSP adherence.
  • Further research is needed to elucidate the complex mechanisms governing MRSP adherence to corneocytes.

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