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Anticandidal activity of biosynthesized silver nanoparticles: effect on growth, cell morphology, and key virulence

Mohammad Jalal1, Mohammad Azam Ansari2, Mohammad A Alzohairy3

  • 1Department of Microbiology, Jawaharlal Nehru Medical College and Hospital, Aligarh Muslim University, Aligarh 202002, India.

International Journal of Nanomedicine
|July 17, 2019
PubMed

Insights

Silver nanoparticles synthesized from Syzygium cumini effectively inhibited Candida species growth and virulence. These nanoparticles suppressed germ tube and biofilm formation, and reduced key enzymes, offering a promising antifungal strategy.

Area of Science:

  • Nanotechnology
  • Mycology
  • Antimicrobial Agents

Background:

  • Candida species exhibit pathogenicity through virulence factors like extracellular enzymes, germ tube formation, and biofilm development.
  • Drug-resistant biofilms pose a significant challenge in treating Candida infections.

Purpose of the Study:

  • To investigate the impact of silver nanoparticles (AgNPs) synthesized from Syzygium cumini (ScAgNPs) on Candida species' growth and virulence attributes.
  • To evaluate the inhibitory effects of ScAgNPs on germ tube formation, biofilm establishment, and extracellular enzyme secretion.

Main Methods:

  • AgNPs were synthesized using Syzygium cumini seed extract and characterized using UV-Vis, FTIR, SEM, EDX, and TEM.
  • Antifungal and antibacterial activities of ScAgNPs were assessed, along with their effects on germ tube and biofilm formation.
  • Inhibition of extracellular enzymes (phospholipases, proteinases, lipases, hemolysin) by ScAgNPs was quantified.

Main Results:

  • ScAgNPs demonstrated significant antifungal activity with Minimum Inhibitory Concentrations (MICs) ranging from 0.125-0.250 mg/ml.
  • ScAgNPs substantially inhibited extracellular enzyme production (up to 82.2% for phospholipases, 83.7% for proteinases) and germ tube formation (up to 97.1%).
  • TEM analysis revealed ScAgNPs penetrated Candida cells, causing damage to the cell wall and cytoplasm, and effectively inhibited biofilm formation.

Conclusions:

  • Biosynthesized ScAgNPs effectively suppressed Candida species' growth, germ tube formation, biofilm development, and secretion of hydrolytic enzymes.
  • ScAgNPs represent a potent agent against Candida virulence factors, warranting further investigation into their molecular mechanisms of action.
Keywords:
LIVE/DEAD staining.ScAgNPsbiofilmgerm tubeshydrolytic enzymesvirulence factors

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