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Updated: Jan 22, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
In vitro antibacterial activity of self-etch bio-active dental adhesives after artificial aging
Gulbike Demirel1, Mujde Eryilmaz2, Hande Seberol3
1Department of Restorative Dentistry, Faculty of Dentistry, Ankara University Turkey.
Purpose:
The aims to evaluate the antibacterial effect of different bioactive component containing dental adhesives before and after artificial aging.
Materials And Methods:
Two bio-active adhesives; Clearfil Protect Bond and FL Bond II, two non-bioactive adhesives, Clearfil SE Bond and Clearfil S3 Bond were used for this study. Antibacterial activities of the fresh and aged samples against Streptococcus mutans were investigated with Direct Contact Test. Data were analyzed with Kruskal Wallis and Mann Whitney U multiple comparison tests.
Results:
For fresh samples FL Bond II and Clearfil Protect Bond exhibit similar antibacterial effect but Clearfil Protect Bond showed significantly higher antibacterial effect after aging the samples (p<0.05).
Conclusion:
The incorporation of bio-active antibacterial components into adhesive systems may be considered as a fundamental component in inhibiting residual Streptococcus mutans when considering the antibacterial effect of fresh samples of bio-active adhesives.
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