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Exploration of potential key pathways and genes in multiple ocular cancers through bioinformatics analysis

Qi Wan1,2, Jing Tang3

  • 1Department of Ophthalmology, The People's Hospital of Leshan, Leshan, 614000, China.

Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
|July 17, 2019
PubMed
Abstract

Insights

This study identified IGF2BP3 as a shared gene across multiple ocular cancers, including retinoblastoma and uveal melanoma. These findings offer new molecular insights into eye cancer development and potential therapeutic targets.

Area of Science:

  • Ophthalmology
  • Oncology
  • Bioinformatics

Background:

  • Primary eye cancers represent a significant clinical challenge in ophthalmology.
  • Understanding the molecular underpinnings of ocular malignancies is crucial for developing targeted therapies.

Purpose of the Study:

  • To explore the molecular mechanisms and identify potential target genes in multiple ocular cancers using a bioinformatics approach.
  • To investigate shared genetic factors across retinoblastoma, uveal melanoma, and basal cell carcinoma of the eyelid.

Main Methods:

  • Downloaded gene expression profiles from the Gene Expression Omnibus (GEO) database (GSE24673, GSE44295, GSE103439).
  • Identified differentially expressed genes (DEGs) using R software and integrated findings with RobustRankAggreg.
  • Performed Gene Set Enrichment Analysis (GSEA) and Gene Ontology (GO) analysis, along with survival analysis for uveal melanoma.

Main Results:

  • Identified 509 DEGs in retinoblastoma, 305 in uveal melanoma, and 753 in basal cell carcinoma.
  • IGF2BP3 was the sole shared DEG across all three ocular cancer types.
  • Enrichment analysis revealed significant involvement of WNT signaling, DNA damage, and apoptotic processes; ID3 and SLC6A15 predicted uveal melanoma survival.

Conclusions:

  • The identification of DEGs and key pathways enhances the understanding of ocular cancer molecular mechanisms.
  • These findings provide novel gene-level insights for future research into cancers of the eye.
  • IGF2BP3 and other identified pathways may serve as potential therapeutic targets for ocular malignancies.
Keywords:
Bioinformatics analysisDifferentially expressed genesGEO databaseOcular cancers

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