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Related Experiment Video

Updated: Jan 22, 2026

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

Purification of Native Flagellin.

María José González¹, Victoria Iribarnegaray¹, Pablo Zunino¹

¹Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.

Methods in Molecular Biology (Clifton, N.J.)

|July 17, 2019

Summary

Researchers developed a method to extract and purify flagellin from Proteus mirabilis. This purified flagellin is crucial for studying its role in bacterial locomotion and virulence.

Keywords:

Flagellum Native flagellin Proteus mirabilis SDS-PAGE Western blot

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Last Updated: Jan 22, 2026

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

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Published on: July 27, 2016

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

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Area of Science:

Microbiology
Molecular Biology
Immunology

Background:

Flagella are essential for bacterial motility and contribute to the virulence of Proteus mirabilis.
Understanding flagellar components is key to deciphering bacterial pathogenesis.

Purpose of the Study:

To establish a protocol for the extraction and purification of native flagellin from Proteus mirabilis.
To provide a reliable method for obtaining flagellin for further research.

Main Methods:

Extraction of native flagellin from Proteus mirabilis.
Purification of flagellin.
Visualization and characterization using SDS-PAGE and immunoblotting.

Main Results:

Successful extraction and purification of native P. mirabilis flagellin.
Demonstrated suitability of purified flagellin for visualization techniques.
Confirmed the utility of purified flagellin for downstream applications like immunization.

Conclusions:

The described protocol provides a method for obtaining pure flagellin from P. mirabilis.
Purified flagellin is a valuable tool for investigating bacterial flagella, motility, and virulence mechanisms.
This purified antigen can be used for developing immunological studies and potential vaccines.