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Adherence of Proteus mirabilis to Uroepithelial Cells

María José González1, Victoria Iribarnegaray1, Paola Scavone1

  • 1Departamento de MicrobiologÚa, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.

Methods in Molecular Biology (Clifton, N.J.)
|July 17, 2019
PubMed

Insights

Proteus mirabilis uses adhesins to attach to host cells, a key step in urinary tract infections. In vitro assays using uroepithelial cells help assess the virulence of these uropathogenic bacteria.

Area of Science:

  • Microbiology
  • Urology
  • Pathogenesis

Background:

  • Bacterial adherence to eukaryotic cells is crucial for infection.
  • Proteus mirabilis frequently causes urinary tract infections, especially in catheterized patients.
  • P. mirabilis possesses numerous fimbrial operons, suggesting significant adherence capabilities.

Purpose of the Study:

  • To describe in vitro adherence assays for P. mirabilis.
  • To evaluate the virulence of uropathogenic P. mirabilis strains.

Main Methods:

  • In vitro adherence assays using P. mirabilis and human uroepithelial cells.
  • Assessment of bacterial attachment to host cells.

Main Results:

  • The study details methods for quantifying P. mirabilis adherence.
  • Results from these assays can differentiate virulence among strains.

Conclusions:

  • In vitro adherence assays are valuable tools for studying P. mirabilis virulence.
  • Understanding adherence mechanisms is critical for combating P. mirabilis infections.
Keywords:
AdherenceProteus mirabilisUrinary tract infectionUroepithelial cellsUropathogenVirulence

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