Actinomadura roseirufa sp. nov., producer of semduramicin, a polyether ionophore

The taxonomic position of 'Actinomadura roseorufa' LMG 30035^T, a semduramicin-producing mutant of strain ATCC 53666^P, which was isolated from a soil sample collected in Yamae Village, Kamamoto, Japan, was clarified in the present study using a polyphasic approach. This Gram-positive, aerobic actinomycete formed a well-developed, extensively branched, non-fragmenting substrate and aerial mycelia which differentiated into single, smooth-appearing spores. Based on analysis of nearly complete 16S rRNA gene sequence, strain LMG 30035^T was found to be closely related to the type strains of Actinomadura fibrosa ATCC 49459^T (98.88 %) and Actinomadura formosensis JCM 7474^T (98.82 %) (pairwise similarity values in parentheses). Digital DNA-DNA hybridisation experiments revealed unambiguously that strain LMG 30035^T represents a novel Actinomadura species (OrthoANIu values less than 83.1 %; dDDH values less than 27.2 % with type strains of validly named Actinomadura species). Analysis of the cell wall revealed the presence of meso-diaminopimelic acid in the peptidoglycan. The whole-cell sugars were glucose, madurose, galactose, ribose and rhamnose. The major polar lipids included phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinones were MK-9(H₆), MK-9(H₈), MK-9(H₄) and MK-9(H₂). The major fatty acids were C_16 : 00, 10-methyl C_18 : 0, C_18 : 1  ω9c and C_18 : 00. The DNA G+C content of its genome was 72.5 mol%. In summary, these characteristics distinguish strain LMG 30035^T from validly named species of the genus Actinomadura, and therefore, we propose to classify this strain formally as the novel species Actinomadura roseirufa sp. nov. with LMG 30035^T (=CECT 9808^T,=ATCC 53664^T) as the type strain.