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Published on: December 1, 2023
Downregulated Long Noncoding RNA PART1 Inhibits Proliferation and Promotes Apoptosis in Bladder Cancer
Xin Hu1,2,3, Hefei Feng1,4,3, Huaxing Huang2,3
11 Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Objective:
In this study, we aimed to clarify the effects of long noncoding ribonucleic acid prostrate androgen-regulated transcript-1 on bladder cancer cell proliferation and apoptosis.
Methods:
Microarrays were implemented to investigate the long noncoding ribonucleic acid expression profiles in bladder cancer tissue (N = 9) and in noncancer bladder tissue (N = 5). Relative prostrate androgen-regulated transcript-1 expression levels in tissue samples or cell lines were detected by real-time quantitative reverse transcription-polymerase chain reaction. Prostrate androgen-regulated transcript-1 expression was enhanced by the transfection of pcDNA3.1-prostrate androgen-regulated transcript-1 and downregulated by the infection with pcMV-sh prostrate androgen-regulated transcript-1. Additionally, cell proliferation and apoptosis were measured by the cell counting kit-8 assay and flow cytometry, respectively. Cell invasion was determined by a Transwell assay.
Results:
Prostrate androgen-regulated transcript-1 expression was upregulated in bladder cancer tissues compared to adjacent nontumor tissues. Furthermore, prostrate androgen-regulated transcript-1 levels were successfully upregulated by pcDNA3.1-prostrate androgen-regulated transcript-1 and depleted by pCMV-sh prostrate androgen-regulated transcript-1 in bladder cancer cell lines (5637, T24). Enhanced prostrate androgen-regulated transcript-1 expression promoted cell proliferation and invasion and inhibited cell apoptosis. However, knockdown of prostrate androgen-regulated transcript-1 expression inhibited cell proliferation and invasion and induced cell apoptosis.
Conclusion:
In summary, these data suggest that the knockdown of prostrate androgen-regulated transcript-1 represents a tumor suppressor player in bladder cancer and contributes to the inhibition of tumor proliferation, the promotion of cell apoptosis, and the suppression of cell invasion. Prostrate androgen-regulated transcript-1 may function as a new prognostic biomarker and as a feasible therapeutic target for patients with bladder cancer.
Insights
Long noncoding RNA prostate androgen-regulated transcript-1 (PCAT-1) promotes bladder cancer progression. Knockdown of PCAT-1 inhibits proliferation and invasion while inducing apoptosis, suggesting it is a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Bladder cancer is a significant health concern with complex molecular underpinnings.
- Long noncoding RNAs (lncRNAs) are emerging as critical regulators in various cancers, including bladder cancer.
Purpose of the Study:
- To investigate the role of lncRNA prostate androgen-regulated transcript-1 (PCAT-1) in bladder cancer cell proliferation and apoptosis.
- To explore PCAT-1 as a potential diagnostic biomarker and therapeutic target for bladder cancer.
Main Methods:
- Microarray analysis to identify lncRNA expression profiles in bladder cancer tissues and adjacent noncancerous tissues.
- Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to measure PCAT-1 expression levels.
- Cell counting kit-8 assay, flow cytometry, and Transwell assay to assess cell proliferation, apoptosis, and invasion, respectively.
Main Results:
- PCAT-1 expression was significantly upregulated in bladder cancer tissues compared to adjacent non-tumor tissues.
- Overexpression of PCAT-1 in bladder cancer cell lines (5637, T24) promoted cell proliferation and invasion while inhibiting apoptosis.
- Knockdown of PCAT-1 expression suppressed cell proliferation and invasion and induced apoptosis.
Conclusions:
- PCAT-1 acts as a tumor suppressor in bladder cancer by inhibiting proliferation, promoting apoptosis, and suppressing invasion.
- PCAT-1 holds promise as a novel prognostic biomarker and a feasible therapeutic target for bladder cancer patients.
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