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Anti-metastasis traditional Chinese medicine monomer screening system based on perinucleolar compartment analysis in

Feifei Liu1, Guohua Lou1, Tianbao Zhang1

  • 1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University Hangzhou 310003, China.

American Journal of Translational Research
|July 18, 2019
PubMed

Insights

Hepatocellular carcinoma (HCC) metastasis is linked to the perinucleolar compartment (PNC). Researchers developed a PNC-based system to screen Traditional Chinese Medicine monomers, identifying effective anti-metastasis compounds.

Area of Science:

  • Oncology
  • Cell Biology
  • Pharmacology

Background:

  • Hepatocellular Carcinoma (HCC) presents a significant challenge due to the lack of effective anti-metastasis drugs.
  • Traditional Chinese Medicine (TCM) monomers show anti-proliferation effects, but specific anti-metastasis agents are scarce.
  • The perinucleolar compartment (PNC) is a nuclear structure implicated in tumor metastasis.

Purpose of the Study:

  • To investigate the correlation between PNC prevalence and HCC metastasis.
  • To establish a screening system for anti-metastasis TCM monomers based on PNC inhibition.
  • To identify effective TCM monomers for inhibiting HCC metastasis.

Main Methods:

  • Correlation analysis of PNC prevalence with HCC patient metastasis and cell line malignancy.
  • Development of a PNC-based screening system using Huh7 cells.
  • Screening of a TCM monomer library to identify compounds reducing PNC prevalence.

Main Results:

  • A positive correlation was observed between higher PNC prevalence and metastasis in HCC tissues and cell lines.
  • Camptothecin (CPT), Evodiamine, and Isoglycyrrhizin were identified as effective PNC inhibitors.
  • The anti-metastatic efficacy of these TCM monomers correlated with their PNC inhibitory activity.
  • CPT demonstrated potential to inhibit Epithelial-Mesenchymal Transition (EMT) by upregulating ZO-1, E-cadherin, and Claudin-1.

Conclusions:

  • The PNC is a potential indicator for HCC metastasis.
  • A novel PNC-based screening system is effective for identifying anti-metastasis TCM monomers.
  • This system offers a promising platform for developing new anti-HCC metastasis drugs.
Keywords:
Hepatocellular carcinomametastasisperinucleolar compartmenttraditional Chinese medicine monomers

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