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An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models
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An Ultrasonic Tool for Nerve Conduction Block in Diabetic Rat Models

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MicroRNA-155 mimics ameliorates nerve conduction velocities and suppresses hyperglycemia-induced pro-inflammatory

Ji Chen1, Wenjie Liu2, Han Yi2

  • 1Department of Endocrinology, The First Affiliated Hospital of University of South China Hunan Province 421001, China.

American Journal of Translational Research
|July 18, 2019
PubMed
Abstract

Insights

MicroRNA-155 (miR-155) mimic treatment improved nerve function and blood flow in diabetic neuropathy (DN) mice. This suggests miR-155 mimic is a potential therapeutic strategy for treating DN.

Area of Science:

  • Endocrinology
  • Neuroscience
  • Molecular Biology

Background:

  • MicroRNA-155 (miR-155) is known to regulate inflammatory cytokines.
  • The specific role of miR-155 in the development of diabetic neuropathy (DN) has not been previously investigated.

Purpose of the Study:

  • To investigate the role of miR-155 in diabetic neuropathy.
  • To evaluate the therapeutic potential of miR-155 mimic in a mouse model of type 2 diabetes.

Main Methods:

  • Expression of miR-155 was analyzed in plasma and sciatic nerves of type 2 diabetic mice (db/db).
  • db/db mice were treated with miR-155 mimic to assess effects on nerve conduction, blood perfusion, and thermal sensitivity.
  • Histological analyses evaluated blood vessel density, intra-epidermal nerve fiber (IENF) count, axon diameter, and myelin sheath thickness.
  • Bioinformatics and microarray analyses identified miR-155 target genes.

Main Results:

  • Diabetic mice exhibited significantly reduced miR-155 levels in sciatic nerves.
  • miR-155 mimic treatment increased miR-155 levels, enhanced sciatic nerve blood flow, and improved motor and sensory nerve conduction velocities.
  • Treatment led to decreased thermal sensitivity threshold, increased blood vessel density, IENF count, axon diameter, and myelin sheath thickness.
  • miR-155 mimic attenuated pro-inflammatory markers (TNF-α, iNOS, IL1-β, Ym1) and decreased expression of TRAF2 and Notch2.

Conclusions:

  • miR-155 mimic treatment demonstrated a therapeutic effect in attenuating diabetic neuropathy in mice.
  • The study identified miR-155 mimic as a potential therapeutic strategy for DN by suppressing associated pro-inflammatory genes.
Keywords:
db/db micediabetic neuropathyinflammatory cytokinesmiR-155 mimic

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