Related Experiment Video

Updated: Jan 22, 2026

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K

Neoantigens Derived from Recurrently Mutated Genes as Potential Immunotherapy Targets for Gastric Cancer

Jie Zhou1, Wenyi Zhao2, Jingcheng Wu1

  • 1Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.

Biomed Research International
|July 18, 2019
PubMed

Insights

Neoantigens offer targeted T-cell immunotherapy for gastric cancer patients with mutated genes. Researchers identified specific neoantigens from recurrently mutated genes as potential therapeutic targets.

Area of Science:

  • Oncology
  • Immunology
  • Genetics

Background:

  • Neoantigens are ideal targets for T-cell immunotherapy, particularly for tumors with undruggable mutations.
  • T-cell immunotherapy presents a potential universal treatment applicable across HLA genotypes sharing common oncogenic mutations.

Purpose of the Study:

  • To identify neoantigens for potential gastric cancer immunotherapy.
  • To analyze somatic mutations and predict neoantigens in gastric cancer patients.

Main Methods:

  • Whole exome sequencing was performed on 32 gastric cancer patients.
  • TSNAD software was utilized to detect somatic mutations and predict neoantigens.
  • Analysis included assessment of mutation patterns and neoantigen load across different tumor stages.

Main Results:

  • High interpatient heterogeneity in somatic mutations was observed.
  • Common mutations included C>A and C>T substitutions, suggesting active nucleotide excision repair.
  • Neoantigen load was significantly higher in stage T1a compared to T2 or T4b.
  • Six recurrently mutated driver genes (PIK3CA, FAT4, BRCA2, GNAQ, LRP1B, PREX2) were identified.

Conclusions:

  • Neoantigens derived from recurrently mutated genes are promising targets for gastric cancer immunotherapy.
  • Further investigation into these neoantigens, considering HLA alleles, is warranted for therapeutic development.

More Related Videos

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells09:24

Combined Conditional Knockdown and Adapted Sphere Formation Assay to Study a Stemness-Associated Gene of Patient-derived Gastric Cancer Stem Cells

Published on: May 9, 2020

4.8K
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer06:21

Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer

Published on: May 10, 2024

1.2K

Related Concept Videos

Mutation, Gene Flow, and Genetic Drift01:09

Mutation, Gene Flow, and Genetic Drift

In a population that is not at Hardy-Weinberg equilibrium, the frequency of alleles changes over time. Therefore, any deviations from the five conditions of Hardy-Weinberg equilibrium can alter the genetic variation of a given population. Conditions that change the genetic variability of a population include mutations, natural selection, non-random mating, gene flow, and genetic drift (small population size).
63.1K
Mutations01:39

Mutations

Overview
94.4K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
8.7K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.6K
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
1.8K
Viral Mutations00:36

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.8K