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Updated: Jan 22, 2026

Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
Effects of different increments in workload and duration on peak physiological responses during seated upper-body
Berit Brurok1,2, Mirjam Mellema3,4, Øyvind Sandbakk5
1Centre for Elite Sports Research, Department of Neuromedicine and Movement Sciences, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Norway. berit.brurok@ntnu.no.
Purpose:
To compare the effects of test protocols with different increments in workload and duration on peak oxygen uptake ([Formula: see text]O2peak), and related physiological parameters during seated upper-body poling (UBP).
Methods:
Thirteen upper-body trained, male individuals completed four UBP test protocols with increments in workload until volitional exhaustion in a counterbalanced order: 20 W increase/every 30 s, 20 W/60 s, 10 W/30 s and 10 W/60 s. Cardio-respiratory parameters and power output were measured throughout the duration of each test. Peak blood lactate concentration (bLapeak) was measured after each test.
Results:
The mixed model analysis revealed no overall effect of test protocol on [Formula: see text]O2peak, peak minute ventilation (VEpeak), peak heart rate (HRpeak), bLapeak (all p ≥ 0.350), whereas an overall effect of test protocol was found on peak power output (POpeak) (p = 0.0001), respiratory exchange ratio (RER) (p = 0.024) and test duration (p < 0.001). There was no difference in POpeak between the 20 W/60 s (175 ± 25 W) and 10 W/30 s test (169 ± 27 W; p = 0.092), whereas POpeak was lower in the 10 W/60 s test (152 ± 21 W) and higher in the 20 W/30 s test (189 ± 30 W) compared to the other tests (all p = 0.001). In addition, RER was 9.9% higher in the 20 W/30 s compared to the 10 W/60 s test protocol (p = 0.003).
Conclusions:
The UBP test protocols with different increments in workload and duration did not influence [Formula: see text]O2peak, and can therefore be used interchangeably when [Formula: see text]O2peak is the primary outcome. However, POpeak and RER depend upon the test protocol applied and the UBP test protocols can, therefore, not be used interchangeably when the latter is the primary outcome parameter.
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