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C7orf59/LAMTOR4 phosphorylation and structural flexibility modulate Ragulator assembly.

Nadia Rasheed1,2, Tatiani B Lima1,3, Gustavo F Mercaldi1

  • 1Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, Brazil.

FEBS Open Bio
|July 18, 2019
PubMed
Summary
This summary is machine-generated.

The HBXIP-C7orf59 dimer is crucial for assembling the Ragulator complex, stabilizing p18 to enable subsequent binding of MP1-p14. This phosphorylation site on C7orf59 regulates p18 interaction, impacting TORC1 signaling.

Keywords:
LAMTORPKARagulatormTORprotein phosphorylationprotein structure

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Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Structural Biology

Background:

  • Ragulator is a pentameric complex essential for amino acid-sensitive TORC1 signaling.
  • It acts as a lysosomal scaffold for Rag GTPases.
  • The components include p18, MP1, p14, C7orf59, and HBXIP (LAMTOR 1-5).

Purpose of the Study:

  • To determine the crystal structure of the human HBXIP-C7orf59 dimer (LAMTOR 4/5).
  • To identify a phosphorylation site on C7orf59 and its role in regulating interactions.
  • To elucidate the sequential assembly mechanism of the Ragulator complex.

Main Methods:

  • X-ray crystallography to determine the structure of the HBXIP-C7orf59 dimer.
  • In vitro phosphorylation assays using protein kinase A (PKA).
  • Cell-based assays in human embryonic kidney 293T cells to study phosphorylation and protein interactions.

Main Results:

  • The crystal structure of the HBXIP-C7orf59 dimer was determined at 2.9 Å resolution.
  • An unfolded N-terminus of C7orf59 (residues 1-15) is essential for p18 binding.
  • Phosphorylation of C7orf59 at Ser67 by PKA modulates its interaction with p18 and is crucial for Ragulator assembly.
  • HBXIP-C7orf59 stabilizes p18, facilitating subsequent MP1-p14 binding.

Conclusions:

  • The HBXIP-C7orf59 dimer acts as a nucleator for the pentameric Ragulator complex.
  • A sequential assembly model is proposed: HBXIP-C7orf59 stabilizes p18, followed by MP1-p14 binding.
  • C7orf59 phosphorylation by PKA plays a regulatory role in Ragulator assembly and TORC1 signaling.