Single-shot phase-sensitive wideband active microrheology of viscoelastic fluids using pulse-scanned optical tweezers

We present a fast phase sensitive active microrheology technique exploring the phase response of a microscopic probe particle trapped in a linear viscoelastic fluid using optical tweezers under an external perturbation. Thus, we experimentally determine the cumulative response of the probe to an entire repertoire of sinusoidal excitations simultaneously by applying a spatial square pulse as an excitation to the trapped probe. The square pulse naturally contains the fundamental sinusoidal frequency component and higher odd harmonics, so that we measure the phase response of the probe over a wide frequency band in a single shot, with the band being tunable over the spectrum by choosing suitable experimental parameters. We then determine the responses to individual harmonics using a lock-in algorithm, and compare the phase shifts to those obtained theoretically by solving the equation of motion of the probe particle confined in a harmonic potential in the fluid in the presence of a sinusoidal perturbation. We go on to relate the phase response of the probe to the complex shear modulus [Formula: see text], and proceed to verify our technique in a mixture of polyacrylamide and water, which we compare with known values in literature and obtain very good agreement. Our method increases the robustness of active microrheology in general and ensures that any drifts in time are almost entirely ruled out from the data, with the added advantage of high speed and ease of use.