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Whole-Genome Sequencing Reveals a Prolonged and Persistent Intrahospital Transmission of Corynebacterium striatum, an

Xuebing Wang1, Haijian Zhou1, Dongke Chen2

  • 1State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, National Institute for Communicable Disease Control and Prevention, Changping, Beijing, China.

Journal of Clinical Microbiology
|July 19, 2019
PubMed

Insights

Genomic analysis of Corynebacterium striatum reveals dominant clones driving hospital-wide multidrug resistance. Understanding this genomic diversity is key to preventing persistent infections in healthcare settings.

Area of Science:

  • Medical Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Corynebacterium striatum is an emerging multidrug-resistant (MDR) pathogen.
  • Its genomic diversity and role in hospital persistence are poorly understood.

Purpose of the Study:

  • To investigate the genomic diversity of C. striatum isolates in a Beijing hospital.
  • To identify the genetic basis of multidrug resistance and hospital-wide dissemination.

Main Methods:

  • Antimicrobial susceptibility testing
  • Pulsed-field gel electrophoresis (PFGE)
  • Whole-genome sequencing (WGS)

Main Results:

  • Nearly all isolates (96.3%) were multidrug-resistant.
  • High resistance rates observed for ciprofloxacin, cefotaxime, and erythromycin.
  • PFGE and WGS identified dominant clones (clades 3a and 4a) responsible for dissemination.
  • Nine resistance genes mobilized by eight unique cassettes were identified.

Conclusions:

  • C. striatum isolates in the hospital are primarily driven by predominant, spreading clones.
  • Rapid adaptation through resistance gene acquisition leads to dominant and persistent clones.
  • Genomic insights are crucial for preventing C. striatum hospital infections.
Keywords:
Corynebacterium striatummultidrug resistancepulsed-field gel electrophoresistransmissionwhole-genome sequencing

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