Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hybrid Zones02:29

Hybrid Zones

21.8K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.8K
DNA Helicases00:55

DNA Helicases

23.9K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
23.9K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

12.4K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
12.4K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.7K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.7K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

9.7K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
9.7K
Hybridization of Atomic Orbitals I03:24

Hybridization of Atomic Orbitals I

66.0K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
66.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Octahedral Dynamics and Local Symmetry in Hybrid Perovskite FAPbI<sub>3</sub> under Thermal Excitation.

ACS omega·2026
Same author

The atypical IκB factor IκBδ enhances CD8 T cell accumulation and effector functions in solid tumors.

bioRxiv : the preprint server for biology·2026
Same author

Folding pathways and force-induced unfolding of neurodegeneration associated GGGGCC microsatellite repeat RNA revealed by molecular simulations.

International journal of biological macromolecules·2026
Same author

Direct Observation of Electric Field-Driven Protein Translocation through the Single-Walled Carbon Nanotube Embedded in a Lipid Bilayer Membrane Revealed a High Dipole Moment of the Transition State.

The journal of physical chemistry. B·2026
Same author

Entropy, Free Energy, and a Generalized Order Parameter for Liquid Crystal Phases of Chiral and Achiral Rods.

Journal of chemical theory and computation·2026
Same author

Meta-analysis of Early Risk Factors Associated with Cerebrovascular Diseases.

Current neurovascular research·2026

Related Experiment Video

Updated: Jan 22, 2026

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
08:42

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Published on: October 26, 2016

12.8K

DNA Translocation through Hybrid Bilayer Nanopores.

Ramkumar Balasubramanian1, Sohini Pal1, Himanshu Joshi2

  • 1Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore 560012, India.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces
|July 19, 2019
PubMed
Summary

Researchers developed a hybrid nanopore system using graphene and DNA origami to control DNA translocation. This system demonstrates base-selective DNA transport through precisely engineered overhangs, enabling controlled DNA sequencing applications.

More Related Videos

Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

31.7K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K

Related Experiment Videos

Last Updated: Jan 22, 2026

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
08:42

Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example

Published on: October 26, 2016

12.8K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

31.7K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K

Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Solid-state nanopores are investigated for DNA translocation control.
  • Pore functionalization is a key strategy for manipulating DNA passage.

Purpose of the Study:

  • To present a hybrid nanopore system for base-selective DNA translocation control.
  • To utilize DNA origami and graphene for enhanced nanopore functionality.

Main Methods:

  • A hybrid nanopore system combining single-layer graphene and a DNA origami layer was designed.
  • Molecular dynamics simulations were employed to optimize origami nanopore and overhang design.
  • The influence of overhang number and spatial distribution on translocation times was analyzed.

Main Results:

  • The hybrid system achieved base-selective control of DNA translocation rate.
  • Specific interactions between origami overhangs and translocating DNA were identified.
  • Simulations revealed base-specific residence times, indicating controlled DNA passage.

Conclusions:

  • The hybrid nanopore system offers a novel approach for base-selective DNA translocation.
  • This technology holds potential for applications in DNA sequencing and analysis.
  • Engineered overhangs on DNA origami provide precise control over DNA-molecule interactions within nanopores.