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Updated: Jan 22, 2026

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
Investigation of Light-Regulated Protein-Protein Interactions Using Yeast Two-Hybrid Assays
1Faculty of Biology, Institute of Biology II, University of Freiburg, Freiburg, Germany. david.sheerin@biologie.uni-freiburg.de.
Abstract:
Yeast two-hybrid allows for investigation of interactions between two proteins in vivo. Yeast media can be supplemented with phycocyanobilin, allowing the formation of photoactive phytochromes, and the investigation of their light-regulated interactions. Three reporter assays are described; the histidine growth selection assay for the HIS3 reporter, and the filter lift and quantitative ONPG assays for the LacZ reporter. Design considerations for yeast two- and three-hybrid experiments are also covered.
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