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Updated: Jan 22, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Characterization of Light-Regulated Protein-Protein Interactions by In Vivo Coimmunoprecipitation (Co-IP) Assays in
Ling Zhu1, Enamul Huq2,3
1Department of Molecular Biosciences, The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, USA.
Abstract:
In light signaling pathways, protein complexes play essential roles in light perception and signal transduction. The phytochrome family of photoreceptors perceives red/far-red region of the light spectrum and then translocates into the nucleus to form protein complexes. Many phytochrome interacting proteins have been identified based on yeast two-hybrid screening and other protein-protein interaction methods. However, it is essential to demonstrate that these proteins interact with phytochromes in vivo to be functionally relevant. In this chapter, a protocol for demonstrating light dependent in vivo interactions between phytochromes and phytochrome interacting proteins is described. This protocol can be adapted for any putative phytochrome interacting protein for validation of their interaction in vivo.
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